Analysis and Visualization Tools

This is a page is a collection of tools we are cataloging as a convenience reference for NWB users. This is not a comprehensive list of NWB tools. Many of these tools are built and supported by other groups, and are in active development. If you would like to contribute a tool, please see the instructions here.

Exploring NWB Files


NWB Widgets is a library of widgets for visualization NWB data in a Jupyter notebook (or lab). The widgets make it easy to navigate through the hierarchical structure of the NWB file and visualize specific data elements. It is designed to work out-of-the-box with NWB 2.0 files and to be easy to extend. Source


NWB Explorer is a web application developed by MetaCell for reading, visualizing and exploring the content of NWB 2 files. The portal comes with built-in visualization for many data types, and provides an interface to a jupyter notebook for custom analyses and open exploration. Online

Extracellular Electrophysiology Physiology Tools


SpikeInterface is a collection of Python modules designed to improve the accessibility, reliability, and reproducibility of spike sorting and all its associated computations. Video tutorial Docs Source


CellExplorer is a graphical user interface (GUI), a standardized processing module and data structure for exploring and classifying single cells acquired using extracellular electrodes. NWB Tutorial Intro Video Video Tutorial Docs Source.


EcogVIS is a Python-based, time series visualizer for Electrocorticography (ECoG) signals stored in NWB files. EcogVIS makes it intuitive and simple to visualize ECoG signals from selected channels, brain regions, make annotations and mark intervals of interest. Signal processing and analysis tools will soon be added. Source.

Optical Physiology Tools


CaImAn is a computational toolbox for large scale Calcium Imaging Analysis, including movie handling, motion correction, source extraction, spike deconvolution and result visualization. CaImAn now supports reading and writing data in NWB 2.0. NWB Demo Video Tutorial Docs Source.


suite2p is an imaging processing pipeline written in Python . suite2p includes modules for 1) Registration, 2) Cell detection, 3) Spike detection, and a 4) Visualization GUI. Video Tutorial Docs Source.


CIAtah (pronounced cheetah; formerly calciumImagingAnalysis) is a Matlab software package for analyzing one- and two-photon calcium imaging datasets. Video tutorial Docs Source.


EXTRACT is a Tractable and Robust Automated Cell extraction Tool for calcium imaging, which extracts the activities of cells as time series from both one-photon and two-photon Ca2+ imaging movies. EXTRACT makes minimal assumptions about the data, which is the main reason behind its high robustness and superior performance. Source NWB tutorials Publication

Intracellular Electrical Physiology Tools


MIES is a sweep based data acquisition tool written in Igor Pro. MIES has three primary user interfaces: 1) the WaveBuilder to generate stimulus sets 2) the DA_Ephys GUI to control and observe data acquisition in real time, and 3) the DataBrowser to browse acquired data. All three interfaces are intended to be operated in parallel. Video tutorial MIES NWB Module Docs Source.



DeepLabCut is an efficient method for 2D and 3D markerless pose estimation based on transfer learning with deep neural networks that achieves excellent results (i.e. you can match human labeling accuracy) with minimal training data (typically 50-200 frames). We demonstrate the versatility of this framework by tracking various body parts in multiple species across a broad collection of behaviors. Documentation

Data Analysis Toolbox


pynapple is a unified toolbox for integrated analysis of multiple data sources. Designed to be “plug & play”, users define and import their own time-relevant variables. Supported data sources include, but are not limited to, electrophysiology, calcium imaging, and motion capture data. Pynapple contains integrated functions for common neuroscience analyses, including cross-correlograms, tuning curves, decoding and perievent time histogram. Docs Source Twitter


Disclaimer: Reference herein to any specific product, process, or service by its trade name, trademark, manufacturer, or otherwise, does not constitute or imply its endorsement, recommendation, or favoring by the NWB development team, United States Government or any agency thereof, or The Regents of the University of California. Use of the NeurodataWithoutBorders name for endorsements is prohibited.